I don't know anyone who is transgender, but I guess the phrase "Ladies and Gentlemen" could make you feel excluded if you were neither.
It costs so little to speak mindfully, and can mean so much to people who could otherwise feel excluded, sidelined, invisible or discriminated against.
TBH it would never have occurred to me about phrases like "Ladies and Gentlemen", being in the privileged position of being securely one gender, but now I am more aware. So thanks for posting this, bazzais.