Did you mean "older un-vaccinated birds" Chris?
If so, that's what happened to us - we bought some replacement pullets from a reputable breeder, and kept them quarantined for three weeks before mixing them with the existing flock. A week later, the original birds started to come down with Mycoplasma (whilst the new ones were unaffected).
We ended up re-homing the new bought-in birds and culling all our existing ones (the Jury's out on whether that was the right thing to do, but we acted on the best information we had at the time). Either way, it was pretty traumatic, and also took us 18 months to get back to where we started. Now, any new hens come in as eggs only.
Ellied - can you find out what these birds have been vaccinated against? It's only certain vaccines that can sometimes cause problems.