Thanks Pete, I did take all of the frames out of the brood boxes so each one was checked visually for her first, then all the bees were brushed off it over the super and then I set that frame aside and moved onto the next frame. Then at the end I put all the frames with brood on and some with hefty stores into one empty brood box and the other frames (either empty or with stores) into the second brood box. I put the brood and stores back on top of the queen excluder and took the second box into another part for the garden, went through it again to remove all bees, separated out the stores frames to put in the freezer for a couple of days and the empty drawn frames stayed in the box for use whenever (some need cutting out and replacing anyway). I can't see how I'd have missed her but I'm sure stranger things have happened!